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The Hammer dataset [@Hammer2010] is a differential gene expression analysis for an experiment with a complex design. Connecticut's premiere virtual tour designer. We used the unnormalized, unfiltered abundances for the analysis. How Stage Is Determined. We will upload all of the images taken directly to Google Street View to get you started. Sınıf Karekod Dinlemeli. Finally, the getResults function returns a binary matrix where rows correspond to features and columns correspond to hypotheses, including the screening hypothesis. To learn the stage of your disease, your doctor may order x-rays, lab tests, and other tests or procedures. stageR provides an automated way to perform stage-wise testing, given p-values for the screening and confirmation stages. Note that the confirmation stage adjusted p-values returned by the function are only valid for the OFDR level provided. This Golf R is built to perfection as any Golf R could be. From the start, we have been focused on top shelf designs, on time deliverables, affordable pricing and outstanding customer service. The ExpressionSet contains the metadata for the samples in pData(esetProstate) and corresponding gene identifiers for the transcripts are stored in fData(esetProstate). Note that the rows in pConfirmation correspond to features (genes) and the features should be identically sorted in pScreen and pConfirmation. Login with your account to access all your game information and compare with friends. Since both control and tumoral tissue are derived from the same patient for all 14 patients, we add a block effect for the patient to account for the correlation between samples within every patient. The dataset is provided with the stageR package and was originally downloaded from the ReCount project website [@Frazee2011]. Studies show that 50% of people are not able to physically visit a property or shop in person. Once you have confirmed your interest and verified your location is photo ready, we can usually get you on the schedule within a week. Want to know more? fects of environmental change on stage-structured (and non-stage struc-tured) species. Similar, the significant transcripts can be returned with getSignificantTx. This can be specified with the method="none" argument. If you only want 360° images for Google Maps, we can provide them. In the TNM (Tumor, Node, Metastasis) system, clinical stage and pathologic stage are denoted by a small "c" or "p" before the stage (e.g., cT3N1M0 or pT2N0). The stage-wise analysis has been adopted from [@Heller2009] and consists of a screening stage and a confirmation stage. How Stage Is Determined. The data is then normalised with TMM normalisation [@Robinson2010] to correct for differences in sequencing depth and RNA population between the samples. Make the most of the short time you have with your visitors by engaging them from the start. Since the Hammer dataset did not require any adjustment, identical results are obtained when manually specifying the adjustments to equal $1$. The significant genes can be returned with the getSignificantGenes function. Tous les postes à pourvoir en une seule recherche. After installing, we will load the package. ® The function stageWiseAdjustment then adjusts the p-values according to a stage-wise analysis. Also pricing was very fair and I will be using them again. Beyond the Stage 4 kit, you can get the more affordable Stage 3 package that limits improvements to the upgraded turbocharger and ECU tune giving your T-Roc R 430 PS (424 HP / … In the confirmation stage, only genes passing the screening stage are considered for analysis. Typically all the photos can be taken in less than 2 hours. The constructor function will check whether the length of pScreen is identical to the number of rows in pConfirmation and return an error if this is not the case. Thanks Dan! The screening hypothesis p-values are then stored in the vector pScreen. There are a few items to address before making your appointment. Ultimate and earlier series entries. A number of FWER control procedures that take into account the logical relations among the hypotheses are implemented. Press J to jump to the feed. The adjusted p-values for genes that did not pass the screening stage are by default set to NA. This period of sleep lasts only a brief time (around five to 10 minutes). My wife and I had the most pleasant and positive experience working with Dan of Stage R Media. The Hammer experiment investigated the effect of a spinal nerve ligation (SNL) versus control samples in rats at two weeks and two months after treatment. In order to avoid this, one should filter these transcripts in the pConfirmation and tx2gene objects. A Game of Thrones stage production is in the early stages of development from producers Simon Painter and Tim Lawson, with the play being written and … We are the company that can deliver. R.R.Q., 1981, c. C-26, r. 189, a. In Stage 1, the brain produces high amplitude theta waves, which are very slow brain waves. In the confirmation stage, every contrast is assessed separately. Note, that the stage-wise adjusted p-values are only valid for the provided significance level and must not be compared to a different significance level. Ethan H. Bob's Little Sport Shop We are an independent design company based in Bristol, CT. Building on over 15 years of experience in the design industry, the company was formed in October of 2014. Kurucusu Zehra Güngör’ün de habercilik bakış açısını kattığı STAGE, o gün de, bugün de içerik yaratma ve haberi ön plana çıkarma anlayışıyla herkesin takdirini topladı. Share on social media right from the virtual tour. Note that when a gene passed the screening hypothesis in the Hammer experiment, only one null hypothesis can still be true: there has to be DE at timepoint 1 or timepoint 2; if the DE only occurs on one timepoint there also exist an interaction; if DE occurs at both timepoints, the $H_0$ of no interaction can still be true. Lesson 10: Double or Two-Phase Sampling We can explore the results of the stage-wise analysis by querying the object returned by stageWiseAdjustment. r/SmashBrosStages: A place to share and discuss custom stages for Super Smash Bros. The more time spent there, the greater chance you have that customers will connect with you. Performing and interpreting both analyses does not provide appropriate FDR control and thus should be avoided. Research and development (R&D, R+D), known in Europe as research and technological development (RTD), is the set of innovative activities undertaken by corporations or governments in developing new services or products and improving existing ones. Or how many times have you left a store and second guessed not buying something because now you're home and you realize it would have been a perfect fit? With time, location and COVID-19 restraints, virtual tours have that ideal convenience factor that allows potential customers to look online before physically visiting a property. r/StarlightStage: Subreddit for the iDOLMASTER Cinderella Girls: Starlight Stage game! Put customers' minds at ease and get them back into your stores. We specialize in interactive 360° virtual tours. The code above is a conventional DEXSeq analysis for analysing differential transcript usage. 305 Followers, 338 Following, 35 Posts - See Instagram photos and videos from Stage R (@stage_r_) 12 Nisan - 17 Nisan * 125 TL üzeri bedava! We can have the virtual ready for presentation within a 10 day time period from the photo-shoot. Any scripts or data that you put into this service are public. Dan was very helpful in making an awesome logo for our company. İngilizce Hikaye Seti (10 Kitap) İlkokul 2. Charles Dickens. Note there has been a typo in the phenoData, so we will correct this first. Either with a simple share of the tour or by contacting you directly, you get additional free advertising or an actual inquiry. It requires a numeric vector of the same length as the number of columns in pConfirmation. What's your time on location? Ce stage est une réelle opportunité, vous aurez la responsabilité d'un secteur d’activité. r/StarlightStage: Subreddit for the iDOLMASTER Cinderella Girls: Starlight Stage game! r/VALORANT: VALORANT is a free to play 5v5, character-based tactical shooter. However, FDR was only controlled at the contrast level and not at the gene level so we cannot state a target FDR level together with this number. There are … r/SmashBrosStages: A place to share and discuss custom stages for Super Smash Bros. 3 Ton 14 SEER 1/6 hp Single-Stage R-410A Split-System Air Conditioner (RA1436AJ1NA) at Ferguson. 125 TL üstü standart teslimatlı siparişlerde kargo bedava! Dan was extremely professional in every aspect of the project. Nobody expects more from us than we do. Re:Stage! Therefore we create a design matrix consisting of two timepoints, two treatments and two biological replicates in every treatment $\times$ time combination. and may either return all p-values or only those from the significant genes, as specified by the onlySignificantGenes argument which can then be ordered or not as specified by the order argument. (Yoriko Tomita, Yasuko Kamo and Tatsuhiko Urahata), and music by Kohta Yamamoto. See the section on Diagnosis to learn more about these tests. After Dan worked his magic the house sold in only four days! İngilizce Türkçe online sözlük Tureng. Connecticut's Premiere Virtual Tour Designer. There are many staging … For more information on customizing the embed code, read Embedding Snippets. KAP'Stages, petites annonces de stages vous propose des milliers d'annonces de stages et de CV stagiaires candidats. stage sahneye koymak stage a protest protestolarla karşılamak ne demek. Privacy | Terms I have a website, some photos and social media. The screening p-values are then adjusted for FDR control after which significance of the screening hypothesis is assessed. Having trouble? p: stage given by histopathologic examination of a surgical specimen; y: stage assessed after chemotherapy and/or radiation therapy; in other words, the individual had neoadjuvant therapy. Panoramic images of your real estate listing, vacation home or business increase the length of stay on your website and social media. Vous êtes à la recherche d'un emploi : Stage Ressources Humaines ? Read through and if you have additional questions, send us an email. The conventional analysis does not find any genes that have a different effect of the treatment between the two timepoints (i.e. It would proceed by either assessing the significant genes according to the gene-wise q-values or by assessing the significant transcripts according to the transcript-level p-values, after adjustment for multiple testing. Il y en a 1 031 disponibles pour Île-de-France sur Indeed.com, le plus grand site d'emploi mondial. Sleep. Nobody expects more from us than we do. The method argument specifies the FWER correction procedure to be used in the confirmation stage. ® Adapt to the new standard and continue to grow your business! Today, Aaron takes me out in his *STAGE 2+* MK7.5 Golf R, and it was insane!! 4.03 . 3 Ton 13 SEER 1/8 hp Single-Stage R-410A Split-System Air Conditioner (4A7A3036H1000N) at Ferguson. Similar as in the DGE example, we port this object to the stageWiseAdjustment function for correcting the p-values. Press question mark to learn the rest of the keyboard shortcuts After which the stage-wise analysis may proceed. Le remboursement moyen demandé pour les clients H&R Block qui ont réclamé un remboursement pendant la saison fiscale 2020 excédait 1 651 $. Ultimate and earlier series entries. Next we build an object from the stageRTx class and indicate that the screening hypothesis p-values were already adjusted by setting pScreenAdjusted=TRUE. This vignette describes how to use the stageR package that has been developed for stage-wise analysis of high throughput gene expression data in R. A stage-wise analysis was shown to be beneficial in terms of biological interpretation and statistical performance when multiple hypotheses per gene are of interest. This can cause an error in the stage-wise analysis, since we have confirmation stage p-values for transcripts but no q-value for their respective genes. www.stageRmedia.com This is a new channel designed to bring you some of the behind the scene antics that go on at stageRmedia.com Look to start your week with us. My wife and I had the most pleasant and positive experience working with Dan of Stage R Media. "STAGE İletişim’i kurumsal anlamda halkla ilişkiler işini ilk yapan firmalardan biri olarak DHL ve Mercedes gibi müşterileriyle tanıdım. Thanks Dan! The adjustment argument from the stageWiseAdjustment function allows the user to specify the FWER adjustment correction. Stage Rh, Région de Rabat-Salé-Kénitra : 132 offres d'emploi disponibles sur Indeed.com. How long do this process take? TL. Research and development constitutes the first stage of development of a potential new service or the production process. Vous serez amené à contacter les Directeurs de sites, service achat, service généraux, services marketing, etc … (centres commerciaux, siège sociaux de grandes sociétés, chaines de commerces, …). Dan nailed it out of the park, and under budget. Search 76 Stage Rh jobs now available on Indeed.com, the world's largest job site. The Muses Go to School: Inspiring Stories about the Importance of Arts in Education : The New Press. The raw sequences have been preprocessed with kallisto [@Bray2016] and transcript-level abundance estimates can be downloaded from The Lair project [@Pimentel2016b] website. Volkswagen’s sporty compact crossover, the T-Roc R, has become a bit more interesting with four tuning options from McChip DKR.. We’ll start with the most potent kit named Stage 4. 75% of the interviewed potential real estate buyers stated that they preferred interactive virtual tours over normal photographs. Our code is based on the formulation by Nisbet and Gurney (1983) us-ing delay differential equations, which are solved using the R-packages deSolveor PBSddesolve. DATE: Saturday, June 19, 2021. Vous êtes à la recherche d'un emploi : Stage Rh ? Faites progresser votre carrière chez ABB avec une équipe mondiale qui alimente la transformation de la société et de l’industrie afin de bâtir un avenir plus productif et durable.Chez ABB, avons l’objectif précis de favoriser la diversité et l’inclusion dans tous ses aspects : genres, LGBTQ+, facultés, ethnicités, générations, etc. He was extremely patient with us and very knowledgeableabout his craft. Stage 2 non-REM sleep – this stage is characterized by a slightly deeper sleep. ® We start by constructing. a: stage determined at autopsy. As the disease progresses, the person with Alzheimer's will require a greater level of care. To get a global picture of the effect of SNL on the transcriptome, we can check how many genes are significantly altered following SNL. All rights reserved. Ethan H. Bob's Little Sport Shop. Hence, the confirmation adjusted p-values returned from this function can be directly compared to the significance level alpha as provided in the stageWiseAdjustment function. Stream Tracks and Playlists from Stage R Records on your desktop or mobile device. So, the stage doesn't change, even though the cancer might. Finally, the pScreenAdjusted argument specifies whether the screening p-values have already been adjusted according to FDR control. Stage Rh, Stagiaire Rh, Offre Stage Communication Rh et bien d'autres : postulez dès maintenant ! Isn't my place too small to have a virtual tour? Thus, according to Shaffer's MSRB procedure [@Shaffer1986], no correction is required in the confirmation stage for this experiment to control the FWER. 4.02; N.I. The first element of the vector is the adjustment for the most significant p-value of the gene, the second element for the second most significant p-value etc. We can then explore the results using a range of accessor functions. For every feature $\times$ hypothesis combination, it indicates whether the test is significant (1) or not (0) according to the stage-wise testing procedure. How do I get started. ProENERG S.A. importator oficial Stager. © Napa EA/MEDX, LLC. Systems that Describe Stage. 87 Meadow St., Unit A1Bristol, CT 06010(by appointment only). Nobody expects more from us than we do. Rheem RA13 Series 13 SEER 1/5 hp Single-Stage R-410A Split-System Air Conditioner www.ferguson.com LEAD LAW WARNING: In accordance with the US Federal or other States laws It is illegal to install products that are not “lead free” certified in potable water systems anticipated for human consumption. Stage Power Packages (261) View All. Body temperature drops and heart rate slows down. Last night I started wondering about ways in which I might be able to use signal processing (Fourier analysis) or symbol dynamics (eg Thinks: Symbolic Dynamics for Categorising Rally Stage Wiggliness?) Our virtual tours can also be embedded directly into your website. L’ESG+ est un guichet unique regroupant la formation et le perfectionnement, la gestion de carrière, les stages et le réseau des diplômés de l’ESG UQAM. 1 Introduction stagePopis an R package that can be used to model the deterministic dynamics Let your customers immerse themselves in the moment and get a true feel for what you're offering. Having a website, and posting photos on social media is a great start. 10,00 TL %35 6,50 TL . See the section on Diagnosis to learn more about these tests. The dataset contains 945 transcripts from 456 genes. 4 Ton 14 SEER 1/5 hp Single-Stage R-410A Split-System Air Conditioner (4TTR4048L1000A) at Ferguson. method="dtu" indicates the adapted Holm-Shaffer FWER correction that was specifically tailored for DTU analysis as described in the manuscript. If this would be of interest, the entire stage-wise testing adjustment should be re-run with the other significance level provided in alpha. The method will return NA p-values for genes with only one transcript if the stage-wise testing method equals "dtu". HILARAPY VIRTUAL STAGE COMEDY SHOW. Hotels that have virtual tours on their website, generate 48% more bookings. No additional software is needed and these virtual tours work with any web enabled device. Transcript-level analyses are supported by the stageRTx class. SEPETE EKLE FİYATI DÜŞÜNCE UYAR. The time on site may vary as it's dependant upon the initial walk through and planning stage. Lesson 9: Multi-stage Designs. İnce Kapak . Meditate. 10pcs/lot 3 PIN AC Powercon Connector Male Plug NAC3FCA NAC3FCB AC Power Plug 20A/250V for Stage Light LED Screen Blue/White R Coaxial Store US $9.03 - 21.15 / lot The game operates on an economy-round, objective-based, first-to-1 … In addition, it utilizes engagement with customers in a way that creates trust and loyalty by providing honest and helpful features. Press question mark to learn the rest of the keyboard shortcuts We will show how to use the stageR package to analyse DTU with a stage-wise approach. Unless you have a photographic memory, this has probably happened to you. We will perform some basic data exploration on the transcripts in the dataset. The output indeed shows that 2 genes and three transcripts are significant because their adjusted p-values are below the specified alpha level of $0.05$. Google's Street View is perfect for simple 360° panoramic images and all the photos we take are compatible with their services. The development version of the package is hosted on GitHub and can be installed with the devtools library using devtools::install_github("statOmics/stageR"). (research by National Assocaiation of Realtors), 65% of travel bookings are researched online. (Re:ステージ!, Risutēji) is a Japanese multimedia franchise by Pony Canyon and Comptiq.It features character designs and illustrations by artist Tsubasu Izumi, series composition and story by "team yoree." Here are the answers to some of our most commonly asked questions. However, our virtual tours outshine the competition with interactive hotspots that display informational windows, menus embedded videos, and make use of directional sound, that will immerse you in the experience. My wife and I opened a recording studio in 2015, and needed a website done. Multiple hypotheses of interest per gene also arise in transcript-level studies, where the different hypotheses correspond to the different isoforms from a gene. Briar Rose. 334 Followers. Audi Stage 2 Power Package A3 FWD/Quattro (8V) $ 1,525.00. Since the logical relations may be specific to the experiment, the user can also specify an adjustment deemed appropriate. For every time $\times$ treatment combination, 2 biological replicates were used. Stager definition, a person of experience in some profession, way of life, etc. APR Stage 3+ 2017 Golf R with the full APR catalog. A subset of the dataset comes with the stageR package and can be accessed with data(esetProstate) after loading stageR. 479 Tracks. The Ren dataset [@Ren2012] analyses differential transcript usage (DTU) in tumoral versus normal tissue in Chinese patients. The stage-wise adjusted p-values are returned using the getAdjustedPValues function. He was on time, extremely polite and knowledgeable, well equipped, and he delivered a stunning product even sooner than we expected would be possible. It's a simple click and go process. We perform indpendent filtering [@Bourgon2010] of the genes and retain genes that are expressed with at least 2 counts per million in 2 samples. How many people have gone apartment shopping or house hunting and almost immediately after a viewing forgot what the space looked like? Relax. Give us a call or send an email so we can discuss what you're looking to accomplish with a virtual tour. 4. We've also use his services for a 3d tour of our facility and will have him back soon for updates. We were referred to Dan by a friend, and asked him to create a website that would look great but also be easy for us to maintain and update. The alpha argument specifies the target OFDR level that is used for controlling the fraction of false positive genes across all rejected genes over the entire stage-wise testing procedure. We start with a regular DEXseq analysis to obtain p-values for every transcript and q-values for every gene. The hypotheses of interest are. r: stage for a recurrent tumor in an individual that had some period of time free from the disease. By default, DEXSeq performs an independent filtering step. getAdjustedPValues returns a matrix where the different rows correspond to transcripts and the respective gene and transcript identifiers are given in the first two columns. The function stageR constructs an object from the stageR class and requires a (preferably named) vector of p-values for the screening hypothesis pScreen and a (preferably named) matrix of p-values for the confirmation stage pConfirmation with columns corresponding to the different contrasts of interest. 5 Ton 16 SEER 1/5 hp Single-Stage R-410A Split-System Air Conditioner (4TTR6060J1000A) at Ferguson. the interaction effect test), while many genes are differentially expressed between treatment and control within every timepoint. Also pricing was very fair and I will be using them again. Line queing, plexiglass barriers and hand sanitizer. More details on the different methods can be found in the help file for stageWiseAdjustment. Online viewers are 115% more likely to book accommodation when a virtual tour or video is viewed. Audi Stage 2 Power Package S3 (8V) $ 1,525.00. Additionally, you'll receive the source images and HTML5 documents to upload the tour on your own website. BeforeTheShoot.pdf. Virtual tours show the personal protection you've put in place to ensure customers have a safe shopping experience. A Game of Thrones stage production is in the early stages of development from producers Simon Painter and Tim Lawson, with the play being written and … We use a contrast for the differential expression at the first and second timepoint and a difference in fold change between the two timepoints, respectively. The third gene in the list is not significant and thus the p-value of the transcript is set to NA. Grab the finest r stage sets that are ideal for weddings, parties, shows and other gatherings at Alibaba.com. Systems that Describe Stage. This may result in a number of genes that have been filtered and thus no q-value for these genes is given in the output of perGeneQValue. Consultez nos 674 offres d'emploi Stage RH à Paris (75) en CDI, CDD ou Intérim publiées sur Optioncarriere. the person may confuse words, get frustrated or angry, and act in unexpected ways, such as refusing to bathe. We are very grateful for his professionalism and high quality work. If a different OFDR level is of interest, the stage-wise testing adjustment of p-values should be re-run entirely with the other OFDR level specified in stageWiseAdjustment. Cancer staging can be divided into a clinical stage and a pathologic stage. Stage R Media Is a great company, Developed an online template that is easy to use and completed the task in a very short amount of time. 40,00 TL %35 26,00 TL . 2016-01-01 (NCPC). Nissan GT-R Stage 3 + CAN Gateway + FF Power Package NIS-008 with TCM Flashing $ 8,125.00. İnce Kapak . How do your virtual tours differ from Google's Street View?   If you awaken someone during this stage, they might report that they were not really asleep. 8.2 - Variance and Cost in Cluster and Systematic Sampling versus S.R.S. Come join us for an afternoon of laughter as our Hilarapy members perform some of their outrageous stand-up sets using comedy to lighten hearts. We had been trying to sell our house for 8 months with no success. For the screening hypothesis, we use the topTableF function from the limma package to perform an F-test across the three contrasts. Fantastic service, very responsive, and very fair. RacingLine Stage 3 Turbo Kit for Golf R, Audi S3, Cupra, GTI upgrade offers 480hp of devastating performance (2.0 TSI OEM+ Stage 3 Turbo Package). All the photos we take can be posted on Google Street View as well as on your social media. This vignette describes how to use the stageR package that has been developed for stage-wise analysis of high throughput gene expression data in R. A stage-wise analysis was shown to be beneficial in terms of biological interpretation and statistical performance when multiple hypotheses per gene are of interest. Une décision du Conseil d’administration imposant un stage ou limitant le droit d’exercice d’un travailleur social stagiaire prend effet 30 jours après son expédition ou sa signification à celui-ci. The adjusted p-values from the confirmation stage can be accessed with the getAdjustedPValues function. We analyse differential transcript usage for a case study that investigated expression in prostate cancer tumoral tissue versus normal tissue in 14 Chinese patients [@Ren2012]. ESG + | Stages . Standart Teslimat. This allows a direct comparison of the adjusted p-values to the provided significance level alpha for both screening and confirmation stage adjusted p-values. The stage-wise analysis first considers an omnibus test that tests whether any of the three contrasts are significant, i.e. Find stage R tour dates, stage R tickets, concerts, and gigs, as well as other events you'll be interested in, only at Last.fm. stageR: stage-wise analysis of high throughput gene expression data in R, stageR: stage-wise analysis of high-throughput gene expression data in R, #if (!requireNamespace("BiocManager", quietly=TRUE)), #nr of significant up-/downregulated genes, stageR: stageR: stage-wise analysis of high throughput gene expression data in R. the treatment effect at the first timepoint, the treatment effect at the second timepoint and, assessing whether the effect of the treatment is different between the two timepoints (i.e. See more. (Research by Best Western). These three objects provide everything we need to construct an instance from the stageRTx class for the stage-wise analysis. Middle-stage Alzheimer's is typically the longest stage and can last for many years. Restez chez vous sauf pour les déplacements essentiels et respectez les restrictions et les mesures de santé publique.. Stay at home except for essential travel … it tests whether there has been any effect of the treatment whatsoever. Transcript-level adjusted p-values for genes not passing the screening stage are set to NA by default. The release version of the package is hosted on Bioconductor, and can be installed with the following code. We will first analyse the data with limma-voom [@Law2014] in a standard way: the three contrasts are assessed separately on an FDR level of $5\%$. In brief, the Holm procedure [@Holm1979] is used from the third transcript onwards and the two most significant p-values are tested on a $\alpha_I/(n_g-2)$ significance level, with $\alpha_I$ the BH adjusted significance level from the screening stage and $n_g$ the number of transcripts for gene $g$. In total, r length(uniqueGenesRegular) genes are found to be differentially expressed following a spinal nerve ligation. It’s {ragg}-time} Automatically Detecting Corners on Rally Stage Routes Using R; How to run Logistic Regression on Aggregate Data in R L’ESG+ est un continuum de services entre l’académique et le monde des affaires et dessert aussi bien les étudiants et diplômés de l’ESG UQAM que les professionnels et dirigeants en poste ou en devenir. Nobody expects more from us than we do. We'd love to hear from you! MK Publications. However, interpretation on the gene level combined with transcript-level results can provide useful biological insights and this can be achieved through stage-wise testing. the treatment-time interaction), a named matrix with transcript-level p-values. Since the dataset was preprocessed for the purposes of this vignette, every gene has at least two transcripts, and all transcripts are expressed in at least 1 sample.

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